Sigma M4655Eagle培養(yǎng)基

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:HEPES: no;NaHCO3: yes;L-glutamine: yes;Earle’s salts (5% CO2): yes;sodium pyruvate: no;

phenol red: yes.

General description:Sigma M4655Eagle培養(yǎng)基Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to c*te normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for c*tion of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Application:Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.