六月，驕陽似火，暑氣留戀。大街上，涌動的人流，五顏六色的衣妝，匯成橫亙天地間的七色彩虹，描繪著生活美不勝收的詩情畫意。

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而就是這樣的六月我們的南京林業(yè)大學(xué)的老師使用上海滬鼎生物自主品牌ELISA試劑盒在《Overexpression of the LcCUC2-like》期刊上發(fā)表的SCI文獻(xiàn)—— gene in Arabidopsis thaliana alters thecotyledon morphology and increasesrosette leaf number順利通過，對生物科研方面有了新的的貢獻(xiàn)。

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【文獻(xiàn)標(biāo)題】Overexpression of the LcCUC2-likegene in Arabidopsis thaliana alters thecotyledon morphology and increasesrosette leaf number

【DOI】Wen et al. (2022), PeerJ, DOI 10.7717/peerj.12615

【作者單位】南京林業(yè)大學(xué) 《Nanjing Forestry University》

【作者】Shaoying Wen1, Jiayu Li1

【產(chǎn)品文獻(xiàn)支持】Auxin content measurement

Basal rosette leaves samples of 0.6 g were harvested from 3-week-old WT and LcCUC2L

transgenic A. thaliana plants and immediately frozen in liquid nitrogen. Determination

of indole-3-acetic acid (IAA) in A. thaliana leaves was performed by ELISA (Huding,

Shanghai, China). Three replicates were used for each sample.

影響因子(IF)= ：2.98

【出版期刊】：《PlantPhysiology and Biochemistry》

【產(chǎn)品原文引用】

Auxin content measurement

Basal rosette leaves samples of 0.6 g were harvested from 3-week-old WT and LcCUC2L

transgenic A. thaliana plants and immediately frozen in liquid nitrogen. Determination

of indole-3-acetic acid (IAA) in A. thaliana leaves was performed by ELISA (Huding,

Shanghai, China). Three replicates were used for each sample.

Histochemical staining to detect GUS activity

The β-Galactosidase Reporter Gene Staining Kit (Leagene) was used for GUS histochemical

staining in ProLcCUC2L transgenic and WT A. thaliana plants. In this study, seven-day-old

seedlings and fourteen-day-old seedlings were processed. Moreover, leaves at different

stages of development (10, 20 and 30 days of age) from plants planted in soil were

subjected to GUS staining. All materials were treated with GUS staining solution and

incubated overnight at 37 ?C, followed by bleaching with 75% (v/v) ethanol.

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