Trans-Blot Turbo轉印系統(tǒng)*結合了傳統(tǒng)轉印技術與現代印跡膜及濾紙合，可在zui短的時間內完成蛋白質從凝膠到膜的轉印，且操作非常簡便。系統(tǒng)內置了高電流電源， 可直接控制鈦陽極和不銹鋼陰極間的電流，能在3分鐘內實現高效快速轉印。全套系統(tǒng)采取即用型設計，研究人員可更快、更輕松的獲得結果，并且具有傳統(tǒng)印跡方 法難以實現的高重復性。
- 快速轉印 — 只需 3 分鐘即可轉印小型凝膠或中型凝膠
- 高通量 — 單次運行可轉印 1–4 塊小型膠或 1–2 塊中型膠
- 更高的轉印效率 — 比其他轉印方法的轉印效率更高
- 靈活設計 — 允許用戶自定義轉移條件并與傳統(tǒng)的半干式消耗品相容
- 環(huán)境友好 — 環(huán)保型耗才可節(jié)省廢棄物處理費用
超高的轉印效率
與其他印跡技術相比較，使用 Trans-Blot® Turbo™ 系統(tǒng)可獲得更高的靈敏度和更好的轉印效率。 這一數據集顯示僅使用 Trans-Blot Turbo 系統(tǒng)成功轉印 1.25 ng 蛋白條帶。 在 Criterion™ TGX™ 4–20% 凝膠上分離轉鐵蛋白的系列稀釋并使用四種不同印跡技術進行轉移。 所有圖像均調整至相同設置。
在 3、7 或 10 分鐘內轉移
使用 Trans-Blot® Turbo™ 轉印包進行快速轉印
3 分鐘方案 — 在短短 3 分鐘內完成 Mini-PROTEAN® TGX™ 凝膠的超快速轉?。ǖ鞍追肿恿糠秶?–150 kD）
7 分鐘方案 — 在 7 分鐘內高效轉印 4塊個小型或 2 塊中型凝膠（蛋白質分子量范圍：5–150 kD）
10 分鐘方案 — 在 10 分鐘內高效轉印 4塊小型或 2 塊中型凝膠（蛋白質分子量范圍：25–300+ kD）
大腸桿菌 裂解物 (6 µg) 稀釋了兩倍。 樣本用 Mini-PROTEAN TGX 凝膠進行分離，用 Trans-Blot Turbo 系統(tǒng)進行轉移，用 SYPRO Ruby 進行染色，然后在 VersaDoc™ MP 4000 系統(tǒng)上進行成像。 通道 1 中的標準品是 Precision Plus Protein™ 未染標準品，頂部帶為 250 kD MW。
更好的轉印不同類型的蛋白
2x 蛋白質轉移對使用 Trans-Blot® Turbo™ 和 iBlot 系統(tǒng)轉印的等量 2-D 凝膠進行的定量表明使用 Trans-Blot Turbo 系統(tǒng)轉印和檢測到的蛋白數增加了一倍。 在 Bio-Rad 的 ReadyStrip™ 11 cm pH 5–8 的 IPG 膠條上聚焦大鼠肝提取物，并將其分離到 AnykD™ Criterion™ TGX™ 凝膠上。 使用 Trans-Blot Turbo 和 iBlot 系統(tǒng)轉移復制凝膠，這兩種系統(tǒng)都使用制造商 ’ *的 7 分鐘實驗方案。 隨后用 SYPRO Ruby 對硝化纖維素膜進行染色，并在 VersaDoc™ MP 4000 系統(tǒng)上進行成像。
| Trans-Blot® Turbo™ Transfer Starter System 1704155 Blotting instrument, includes base, 2 cassettes to hold 1–2 midi or up to 4 mini blotting sandwiches, blot roller, and starter consumable kit | Trans-Blot® Turbo™ Transfer System 1704150 Blotting instrument, includes base, 2 cassettes to hold 1-2 midi or up to 4 mini blotting sandwiches, blot roller | ||
| Trans-Blot® Turbo™ Mini PVDF Transfer Packs 1704156 Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system | Trans-Blot® Turbo™ RTA Mini PVDF Transfer Kit, for 40 blots 1704272 Ready-to-assemble transfer kit includes 40 mini-sized PVDF membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks | ||
| Trans-Blot® Turbo™ Midi PVDF Transfer Packs 1704157 Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system | Trans-Blot® Turbo™ RTA Midi PVDF Transfer Kit, for 40 blots 1704273 Ready-to-assemble transfer kit includes 40 midi-sized PVDF membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks | ||
| Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs 1704158 Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system | Trans-Blot® Turbo™ RTA Mini LF PVDF Transfer Kit, for 40 blots 1704274 Ready-to-assemble transfer kit includes 40 mini-sized LF PVDF membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks | ||
| Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs 1704159 Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system | Trans-Blot® Turbo™ RTA Midi LF PVDF Transfer Kit, for 40 blots 1704275 Ready-to-assemble transfer kit includes 40 midi-sized LF PVDF membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks | ||
| Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit, for 40 blots 1704270 Ready-to-assemble transfer kit includes 40 mini-sized nitrocellulose membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks | Trans-Blot® Turbo™ Cassette 1704151 Pkg of 1, cassette, to hold 1 midi or up to 2 mini blotting sandwiches, for use with Trans-Blot Turbo transfer system | ||
| Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit, for 40 blots 1704271 Ready-to-assemble transfer kit includes 40 midi-sized nitrocellulose membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks | Trans-Blot® Turbo™ Base 1704152 Blotting instrument base, does not include any cassettes |
耗材試劑套裝：
Use the Trans-Blot Turbo RTA transfer kit for fast, efficient protein transfer with the Trans-Blot Turbo rapid western blotting transfer system.
- Economical transfer option for high-throughput labs
- Optimized buffer, membrane, and filter paper combination
- PVDF membrane reduces nonspecific binding
- For 40 mini-gel transfers; requires assembly
Related Products
View our wide selection of transfer membrane sandwiches for the Trans-Blot Turbo system:
- Preassembled transfer packs for 10 transfers
- Ready-to-assemble kits for 40 transfers
- PVDF, low fluorescence (LF) PVDF, or nitrocellulose membranes
| Trans-Blot Turbo Transfer Packs and Kits | Preassembled Transfer Packs (10 Transfers) | Ready-to-Assemble Transfer Kits (40 Transfers) | ||
| Membrane Type | Mini | Midi | Mini | Midi |
| 0.2 µm PVDF | 1704156 | 1704157 | 1704272 | 1704273 |
| 0.45 µm LF (low fluorescence) PVDF | — | — | 1704274 | 1704275 |
| 0.2 µm Nitrocellulose | 1704158 | 1704159 | 1704270 | 1704271 |
TGX凝膠訂購信息：
| Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl 4569033 Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells | 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl 4561093 Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells | ||
| 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl 4561083 Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells | 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl 4561096 Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells | ||
| 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl 4561086 Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells | 10% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl 4561033 Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells | ||
| 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl 4561023 Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells | 12% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl 4561043 Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells | ||
| 10% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl 4561036 Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells | 12% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl 4561046 Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells |
